In order to detect terbutaline (TB) quickly and sensitively, a nuclear-satellite nanostructured Fe₃O₄/SiO₂/Au-MNPs magnetic substrate was prepared for surface enhanced Raman spectroscopy (SERS) detection. The linear relationship between the concentration of terbutaline and the intensity of the Raman spectrum was investigated by magnetic separation andpH adjustment of the system and a calibration curve was drawn. The experimental results showed that the detection limit of TB using the core-satellite magnetic SERS substrate was 3.77 × 10^-10 mol/L. The detection had a linear relationship with the concentration range from 5×10^-5 mol/L to 5×10^-9 mol/L. The linear correlation coefficient R² obtained by least square fitting was 0.996. The preparation method of the composite material was simple and easy, which provided inspiration for the synthesis of other nanocomposite materials.