Visible and near-infrared (VNIR) spectroscopy is an eco-friendly method used for estimating plant nutrient deficiencies. The aim of this study was to investigate the possibility of using VNIR method for estimating Zn content in cherry orchard leaves under field conditions. The study was conducted in 3 different locations in Isparta region of Turkey. Fifteen cherry orchards containing normal and Zn deficient plants were chosen, and 60 leaf samples were collected from each location. The reflectance spectra of the leaves were measured with an ASD FieldSpec HandHeld spectroradiometer and a plant probe. The Zn contents of leaf samples were predicted through laboratory analysis. The spectral reflectance measurements were used to estimate the Zn levels using stepwise multiple linear regression analysis method. Prediction models were created using the highest coefficient of determination value. The results show that Zn content of cherry trees can be estimated using the VNIR spectroscopic method (87.5