Laser & Optoelectronics Progress, Volume. 54, Issue 12, 123001(2017)
Effect of Environmental Factors on Staphyloxanthin Biosynthesis Based on Laser Tweezers Raman Spectroscopy
Laser tweezers Raman spectroscopy (LTRS) is used to study the effects of various environmental factors on staphyloxanthin biosythesis. Staphylococcus aureus cells are cultivated at the temperature ranging from 22 ℃ to 37 ℃, pH from 4.5 to 8.5, glucose mass concentration from 0 g/L to 10.0 g/L, and flavone mass concentration from 0 mg/L to 175 mg/L. The intensity of Raman band at 1523 cm-1 is used to characterize the relative content of staphyloxanthin in bacterial cells. The experimental results show that the temperature ranging from 22 ℃ to 37 ℃ has no significant effect on staphyloxanthin biosynthesis, the neutral pH is most favorable for staphyloxanthin biosynthesis, and glucose in a certain mass concentration range (0.2-5.0 g/L) promotes staphyloxanthin synthesis in a dose-dependent manner whereas flavone inhibits staphyloxanthin synthesis in a dose-dependent manner. The LTRS technology can serve as a rapid and reliable method for quantitative determination of staphyloxanthin content as well as an effective approach to screening and evaluating inhibitors of staphyloxanthin biosynthesis.
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Tao Zhanhua, Ke Ke, Shi Deqiang, Zhu Libo. Effect of Environmental Factors on Staphyloxanthin Biosynthesis Based on Laser Tweezers Raman Spectroscopy[J]. Laser & Optoelectronics Progress, 2017, 54(12): 123001
Category: Spectroscopy
Received: May. 26, 2017
Accepted: --
Published Online: Dec. 11, 2017
The Author Email: Zhanhua Tao (taozhanhua@163.com)