The transmittance spectra and Fluorescence spectra of Eu3+:Y2SiO5 crystal have been studied at room temperature. Spectral analysis shows that two optical sites with low symmetry were occupied by Eu3+ in this crystal. Spectra of two kinds of C1 sites were resolved at 5D0 level by transmittance spectrum and fluorescence spectrum. There is 0.18 nm space between them, and the spectral linewidths of each site are about 0.1 nm. In addition, some of the level structure of 7FJ(J=0, 1, 2, 3, 4), 5D1, 2 and 5L6 were also observed.