Spectroscopy and Spectral Analysis, Volume. 35, Issue 11, 3111(2015)
The Thiacalix[4]arene-Coumarin Fluorescence Probe Recognition for Fe3+ and Bovine Hemoglobin
1,3-alternate coumarin substituted thiacalix[4]arene fluorescent probe 1 was synthesised from 1,3-alternate diethyl thiacalix[4]arene and 7-hydroxycoumarin by-step reactions. In DMSO/H2O (φ, 3/7, pH 7) solution, the strong fluorescence emission and UV absorption of probe 1 can be selectively quenched or significantly enhanced by Fe3+ ion. The probe 1 showed high Fe3+ selective fluorescence quenching or absorption enhancement over commonly coexistent metal ions in neutral aqueous media, and the limit of detections were obtained as low as 10-8 mol·L-1 of Fe3+ by fluorescence and absorption spectrometry. Spectral titration, isothermal titration calorimetry and mass spectrometry were showed that probe and Fe3+ form 1∶1 complexes, the constant up to 105 L·mol-1 and coordinate process was spontaneous by the mole binding free energy and entropy of probe with Fe3+. In addition, the probe can identification bovine hemoglobin (BHb) over other proteins through quenched its fluorescence in DMSO/H2O (φ, 1/9, Tris-HCl, pH 7, 0.1 mol·L-1 NaCl) media. The limit of detection was obtained as 0.12 μg·mL-1 of BHb, as well as a linearity of 0.2~3.0 μg·mL-1, indicating the probe of high sensitivity and quantitation range. It can be used as a selective recognition Fe3+ and BHb of thiacalix<参考文献原文>arene fluorescent probe.
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LI Yan-qin, MU Lan, ZENG Xi, LI Jun, Takehiko Yamato. The Thiacalix[4]arene-Coumarin Fluorescence Probe Recognition for Fe3+ and Bovine Hemoglobin[J]. Spectroscopy and Spectral Analysis, 2015, 35(11): 3111
Received: Jun. 13, 2014
Accepted: --
Published Online: Feb. 2, 2016
The Author Email: Yan-qin LI (821597431@qq.com)