Novel glass-ceramics containing Cu2O nanocrystallines with features of high Cu loading amount, low cost and easy large scale production is a potential substitute for silver loaded antibacterial glass. In this study, SiO2-Al2O3-K2O-ZnO-P2O5-B2O3-CuO glass-ceramics with different ZnO/K2O were investigated. Their microstructures were characterized by XRD, Raman spectroscopy, XPS, FESEM and TEM. The structure-performance relationship was also discussed. The results indicate that there is an enrichment of Zn and P elements around the region where Cu element locates in the glass-ceramics. An appropriate amount of ZnO makes sure the particles size of Cu2O crystallines precipitated in glass-ceramics be in nano-scale and regulate the leaching rate of Cu element. Glass-ceramics containing Cu2O nanocrystallines exhibit significant antibacterial effects on Escherichia coli and Staphylococcus aureus. They also show photocatalysis activity for degradation of Victoria blue B solution under visible light. It should be a novel functional glass-ceramics material with great potential.