A novel chemiluminescence method coupled with flow injection technique for the determination of ractopamine was developed. It was based on the enhancement of the chemiluminescence by ractopamine derived from the chemiluminescence reaction between luminol and ferricyanide in sodium hydroxide medium. The linear calibration range of the chemiluminescence intensity with respect to the ractopamine concentration covers from 4.0×10-9～8.0×10-7 g·mL-1. The relative standard deviation for ractopamine is 5.6% (n=11), and the detection limit is 2.5×10-9 g·mL-1. The method was firstly applied to the determination of ractopamine in biological samples with satisfactory results. The recovery was between 69.3% and 101.3%.