Acta Optica Sinica, Volume. 39, Issue 7, 0718001(2019)
Improvement of Imaging Resolution of Fluorescence Emission Difference Super-Resolution Microscopy with Annular Pupil
Super-resolution imaging can be achieved by fluorescence emission difference (FED) microscopy through the subtraction of a negative confocal image scanned by a hollow focal spot from a confocal image scanned by a solid focal spot. This study proposes an easily accessible annular pupil filter to enhance the resolution of deformation-free FED imaging. The hollow spot size is reduced by an appropriate annular pupil filter in the light path of hollow spot. Meanwhile, a diaphragm with a high numerical aperture is permitted for the beam of a solid focal spot, and a small solid focal spot which is matching well with the hollow focal spot is achieved. The spatial resolution of FED microscopy is increased under the effects of these two aspects.
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Suhui Deng, Jie Hu, Yiping Xiao, Mingping Liu. Improvement of Imaging Resolution of Fluorescence Emission Difference Super-Resolution Microscopy with Annular Pupil[J]. Acta Optica Sinica, 2019, 39(7): 0718001
Category: Microscopy
Received: Jan. 23, 2019
Accepted: Mar. 21, 2019
Published Online: Jul. 16, 2019
The Author Email: Liu Mingping (mpliu@ncu.edu.cn)