Spectroscopy and Spectral Analysis, Volume. 37, Issue 11, 3475(2017)
Determination of Cephradine Capsules Content Based on Fluorescence Quenching Method of FOS-μSIA-LOV
The content of cephradine capsules was determined by a system of FOS-μSIA-LOV(fiber optic sensor-micro sequential injection-valve laboratory), based on the principle of cefradine quenching fluorescein in methanol solvent, optimize experimental conditions. The order of inhalation was anhydrous methanol, cefradine, fluorescein and anhydrous methanol; the carrier liquid suction volume was 500 μL; cefradine inhalation volume was 100 μL. Cefradine quenched fluorescein fluorescence intensity and concentration in 0.05~0.5 mg·mL-1 linear relationship as ΔF=812.6+284.41, r=0.999 5, precision RSD was 4.4%, the recovery was 101%~103%. FOS-SIA-LOV and personalized drug testing and programming resulted in automated on-line process analysis with the good reproducibility, less reagent consumption, time and labor saving.
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QIU Xin, LI Xin-xia, Arkin IBURAIM, Gulina KUERBAN, ZHANG Li-hua, LI Yan. Determination of Cephradine Capsules Content Based on Fluorescence Quenching Method of FOS-μSIA-LOV[J]. Spectroscopy and Spectral Analysis, 2017, 37(11): 3475
Received: Jan. 11, 2016
Accepted: --
Published Online: Jan. 4, 2018
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