Contents of 21 elements, including of K, Ca, Mg, Na, Fe, Mn, Cu, Zn, Ni, Ti, Cr, Mo, V, Zr, Co, Se, Sn, Cs, Cd, Hg, and Pb, in the roots of four germplasms Bupleurum L. planted in the soils from seven regions, were analyzed by ICP-MS/ICP-AES. The results showed that the contents of K, Ca, Mg and Na are mainly ascribed to the effect of germplasms, and the contents of Fe, Cu, Ni, Ti, Cr, V, Zr, Se, Sn, Cs, Cd, Hg and Pb are mainly ascribed to the effect of soils considering the integrated effects of germplasms and soils. Generally, element contents in the root of G-GS were higher and that in the root of G-ZC were lower in the four germplasms of Bupleurum L. Element contents in the root of Bupleurum L. planted in S-HR were higher, while that planted in S-YQS were lower as a whole among these soils from seven regions. The difference in the element contents has an important effect on the research into the contents of officinal ingredients and their curative effect in the roots of four original Bupleurum L. germplasms planted in the soils from seven regions. And this study also provides an important scientific foundation for the choices of germplasms and soils in the cultivation of high-yield and high-quality Bupleurum L.