It briefly describes the radio-frequency identification technology (RFID) middleware and its current research, based on the study and analysis of the limitations and inadequacies of the existing food tracing system; it designs a layered RFID middleware architecture, and conducts an in-depth study. Layered middleware shields the differences of different devices, centralizes to manage distributed reader devices, according to a protocol rule to filter large amounts of the redundant data; and extract semantic information using publish-subscribe model for the more efficient, real-time information sharing mechanism.