Pulse driving and controlling technology of micro-fluids was introduced. The pulse driving and controlling process of micro-fluids was analyzed in detail and it points out that the acceleration of micro channel solid wall and the viscosity force of micro-fluidic are the main factors that influence on the flow of the micro-fluids. A method of "elliptic rounding" was adopted to round driving voltage waves. As the driving directions of the micro-fluids were determined by the locations of rounding points, a series of waves with different rounding locations and rounding coefficients were obtained. The influence rules of rounded waves on the acceleration of micro channel solid wall, micro-fluidic pulse inertial force and driving effects were researched by experiments. The experimental result indicates that the flow rate of micro fluidic volume can be in the range of 0~15.4 pl/min, which is more better than that of the current micro driving technology. The research results can provide a reference for the research on the further application of pulse driving and controlling technology of micro-fluids in micro-fluidic systems.