Journal of Innovative Optical Health Sciences, Volume. 14, Issue 3, 2150008(2021)

Simulation of stimulated Raman scattering signal generation in scattering tissues excited by Bessel beams

Xinyu Wang1,2, Lin Wang3, Peng Lin4, Hui Xie1,2, Xinyi Xu1,2, Qi Zeng1,2, Yonghua Zhan1,2, and Xueli Chen1,2、*
Author Affiliations
  • 1School of Life Science and Technology Xidian University, Xi'an, Shaanxi 710126, P. R. China
  • 2Engineering Research Center of Molecular & Neuro Imaging of the Ministry of Education Xi'an, Shaanxi 710126, P. R. China
  • 3School of Computer Science and Engineering Xi'an University of Technology Xi'an, Shaanxi 710048 P. R. China
  • 4Department of Electrical & Computer Engineering Boston University, Boston Massachusetts 02215, USA
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    Stimulated Raman scattering (SRS) microscopy has the ability of noninvasive imaging of specific chemical bonds and been increasingly used in biomedicine in recent years. Two pulsed Gaussian beams are used in traditional SRS microscopes, providing with high lateral and axial spatial resolution. Because of the tight focus of the Gaussian beam, such an SRS microscopy is difficult to be used for imaging deep targets in scattering tissues. The SRS microscopy based on Bessel beams can solve the imaging problem to a certain extent. Here, we establish a theoretical model to calculate the SRS signal excited by two Bessel beams by integrating the SRS signal generation theory with the fractal propagation method. The fractal model of refractive index turbulence is employed to generate the scattering tissues where the light transport is modeled by the beam propagation method. We model the scattering tissues containing chemicals, calculate the SRS signals stimulated by two Bessel beams, discuss the influence of the fractal model parameters on signal generation, and compare them with those generated by the Gaussian beams. The results show that, even though the modeling parameters have great influence on SRS signal generation, the Bessel beams-based SRS can generate signals in deeper scattering tissues.

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    Xinyu Wang, Lin Wang, Peng Lin, Hui Xie, Xinyi Xu, Qi Zeng, Yonghua Zhan, Xueli Chen. Simulation of stimulated Raman scattering signal generation in scattering tissues excited by Bessel beams[J]. Journal of Innovative Optical Health Sciences, 2021, 14(3): 2150008

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    Paper Information

    Received: Oct. 22, 2020

    Accepted: Jan. 11, 2021

    Published Online: Aug. 6, 2021

    The Author Email: Chen Xueli (xlchen@xidian.edu.cn)

    DOI:10.1142/s1793545821500085

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