A set of RF-electrostatic coupling power supply circuit for segmented linear ion trap is designed for trapping ions and micromotion compensation. The trapping of calcium ions in ion trap is realized. The ion crystal position is determined by observing the fluorescence emitted by ions with CCD camera. The precise control of ion crystal in three-dimensional space is realized by controlling the static voltage on the 12-segment poles in ion trap independently, which reduces the micromotion caused by RF modulation. The ions trapping system can further determine the physical properties of ion crystals, such as the secular motion frequency of ions in ion trap, temperature distribution of ion crystal, and the trapping of multi-component ion crystal.