The excitation of bioluminescence by different flow regimes generated with a Couette chamber was examined using the dinoflagellates Pyrocystis noctiluca, Electron-Multiplying CCD was used to detect at a fixed position. In this paper, luminescence characteristics of P. noctiluca were qualitatively studied under different flow field stimulation. An open water tank containing a certain concentration of P. Noctiluca was used to simulate the sea surface environment, and a water model with a propeller was used to make counterclockwise circular motion. The image data obtained from EMCCD was processed and analyzed based on the single-scale Retinex algorithm, so as to maintain the maximization of optical wake characteristics and suppress the influence of the surface background. After image enhancement, the wake of target motion can be clearly observed to rotate counterclockwise, which preliminarily proves that the visualization of target motion trajectory in water can be realized by using the excitation light phenomenon of Marine organisms. Therefore, biologically stimulated light can be used as a new technique and method to detect moving objects in the ocean.