In this paper, we present a three-parametric model using Raman and fluorescence spectroscopy for detection and analysis of esophagus dysphasia and esophagus cancer. The model was set up from more than 1000 samples. And 56 samples were used to test this algorithm of the model prospectively. The serum spectra were excited by laser of the wavelengths 488.0 and 514.5 nm. The apparent differences of auto-fluorescence and Raman spectroscopy were observed for patients compared to the normal: the majority of the fluorescence spectra did not have violent alteration, but three Raman peaks disappeared or were found to be very weak. Moreover, 'Delta lambda' value (red shift of fluorescence peak) and 'alpha'-value (rate of fluorescence intensity) also provide the reference for future research. And I-value (intensity of Raman peak) will decrease with progression of the tumor. The result of spectral analysis is accordance with the clinical diagnosis.