Chinese Optics Letters, Volume. 11, Issue 11, 111102(2013)
Label-free biomolecular imaging using scanning spectral interferometry
This letter presents a label-free biomolecular imaging technique based on white-light interferometry and spectral detection. The method measures thickness changes caused by specific binding between biomolecules to detect the presence of certain analyte. A spectrum-shifting algorithm is developed to resolve the thickness information from the spectrum. The axial resolution of the experimental instrument can reach ~1 nm, thereby enabling detection of trace amounts (~1 ng/mm2) of proteins or DNA. This letter also presents two experiments to prove the feasibility of the method for detecting proteins and DNA without fluorescent labeling.
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Tongzhou Wang, Liping Xie, Haley Huang, Xin Li, Ruliang Wang, Guang Yang, Yanan Du, Guoliang Huang. Label-free biomolecular imaging using scanning spectral interferometry[J]. Chinese Optics Letters, 2013, 11(11): 111102
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Received: Jul. 16, 2013
Accepted: Oct. 17, 2013
Published Online: Dec. 6, 2013
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