With the widely application of 1394 bus in the field of airborne, taking account of low cost and flexibility, a complete set of 1394 bus signal quality testing system is needed to be established for the process of configuration, simulation, testing, monitoring and test. The signal quality testing for product module level and system level can be realized by the reliable bus signal quality testing system, at the same time, the development cycle of the system is shortened and the development cost is saved greatly. A 1394 bus signal quality testing system design scheme is proposed. Based on the full understanding of 1394 bus protocol, the hardware, software and testing architectures, the design and implementation of 1394 bus signal quality testing system are introduced, which can provide reliable technical guarantee for the development and verification of the airborne equipment based on 1394B bus communication.