A multi-channel temperature testing system based on MSP430 is researched. Temperature changing states of 36 points on a measured object can be measured and stored by the system. And the data can be transmitted through radio in real time. Finally, changing curves of stored temperature are displayed in real time. A multi-channel temperature testing system is designed to solve some problems as followed. The reliabilities of data are ensured by encoding the data. The accuracy of data transmission is ensured by adopting time-sharing transmission data. The continuity and accuracy of data storage are ensured by adopting alternately storage mode with two pieces of FLASH. The data decoding and error data detection are implemented. The data transmission in real time through universal serial bus (USB) interface is implemented and data of temperature testing system is read back.