To resolve the calibration of temporal and spatial nonlinearity of an optical streak camera in 100-nanosecond level, an idea of rapid calibration of two-dimensional nonlinearity of optical streak camera has been proposed with the method of constructing an equidistant standard light field in the temporal and spatial dimension. The reference points with equal spacing of temporal dimensions have been obtained by the technique of sequential pulsed light construction. In addition, the reference points with equal spacing of spatial dimensions have been obtained by the spatial modulation technique of light field. The design criteria of sequential pulsed light and optical system have been analyzed. Based on the criteria, a high-performance system to calibrate two-dimensional nonlinearity of optical streak camera has been developed. The temporal resolution of the system is 0.1 ns and the spatial resolution is less than 90 μm. Based on the system, the sweeping nonlinearity, time dispersion, temporal distortion and geometric aberration of a domestic streak camera have been calibrated quickly via a single sweeping image. In addition, the sweeping nonlinearity of different circuits has been analyzed and verified by the system. The calibration system quickly verified that the double-sided scanning circuit was conducive to optimizing the scanning nonlinearity. The analysis and verification results show that the calibration technology could calibrate the nonlinear parameters of a streak camera via a single sweeping image.