Acta Optica Sinica, Volume. 39, Issue 12, 1218001(2019)
Microscopy Autofocus Method Using Tilt Camera
To overcome the disadvantages of the existing microscopy autofocus technology, this study proposes a method for detecting the defocusing distance and orientation of a sample according to the distance and direction from the center of the field of view (FOV) of the focusing band in a tilt plane image, which is acquired using a tilt camera. The feasibility of this method is verified by constructing an experimental microscope autofocus system. Results show that this system can quickly obtain a defocusing distance and orientation by detecting the amount of horizontal shift from the center of FOV of the focusing band in a single-frame tilt plane image, which can be determined according to a calibration curve between the defocusing distance and focusing band's horizontal-shift value. Autofocus can then be achieved in applications using motor drive. The optical structure presented herein is simple and cost-effective. It can be directly used in a conventional, trinocular microscope-imaging system and can thus be applied to the autofocus process required in online, in situ, large FOV, and high-resolution detection in various fields.
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Yiqing Ye, Dingrong Yi, Yongzhen Zhang, Linghua Kong, Wei Jiang. Microscopy Autofocus Method Using Tilt Camera[J]. Acta Optica Sinica, 2019, 39(12): 1218001
Category: Microscopy
Received: May. 31, 2019
Accepted: Sep. 2, 2019
Published Online: Dec. 6, 2019
The Author Email: Yi Dingrong (yidr@hqu.edu.cn)