Spectroscopy and Spectral Analysis, Volume. 32, Issue 12, 3175(2012)
Visualization of the Age-Related Changes in Expressions of DNA Methyltransferases in Mouse Oocytes Using Two-Photon Imaging System
Quantum dot (QD) is widely used as fluorescent labeling dye for its strong anti-fluorescence quenching, high quantum yield, wide absorption spectra, and narrow emission spectra. In the present paper, QD 585-labeled DNA methyltransferases (Dnmts) and Hoechst 33342-labeled chromosomes were imaged simultaneously using two-photon imaging system. The authors’ results showed that aging mouse oocytes may be not suitable for in-vitro maturation: both the localizations and expression levels of Dnmts in in-vitro matured oocytes of aging mice were changed, and these changes may be related to the abnormal DNA methylation modification.
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TIAN Ning, ZHANG Lu, ZHENG Jing-hao, LI Ying, MA Wan-yun. Visualization of the Age-Related Changes in Expressions of DNA Methyltransferases in Mouse Oocytes Using Two-Photon Imaging System[J]. Spectroscopy and Spectral Analysis, 2012, 32(12): 3175
Received: Apr. 19, 2012
Accepted: --
Published Online: Jan. 14, 2013
The Author Email: Ning TIAN (tiann517@yahoo.cn)