Journal of Innovative Optical Health Sciences, Volume. 5, Issue 3, 1250018(2012)

RECENT PROGRESS IN MULTIFOCAL MULTIPHOTON MICROSCOPY

JUNLE QU1、*, LIXIN LIU2, YONGHONG SHAO1, HANBEN NIU1, and BRUCE Z. GAO3
Author Affiliations
  • 1College of Optoelectronic Engineering, Shenzhen University Key Laboratory of Optoelectronic Devices and Systems of Ministry of Education and Guangdong Province, Shenzhen 518060, P. R. China
  • 2School of Technical Physics, Xidian University Xi'an 710071, P. R. China
  • 3Department of Bioengineering and COMSET Clemson University, Clemson SC 29634, USA
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    Multifocal multiphoton microscopy (MMM) has recently become an important tool in biomedicine for performing three-dimensional fast fluorescence imaging. Using various beamsplitting techniques, MMM splits the near-infrared laser beam into multiple beamlets and produces a multifocal array on the sample for parallel multiphoton excitation and then records fluorescence signal from all foci simultaneously with an area array detector, which significantly improves the imaging speed of multiphoton microscopy and allows for high efficiency in use of the excitation light. In this paper, we discuss the features of several MMM setups using different beamsplitting devices, including a Nipkow spinning disk, a microlens array, a set of beamsplitting mirrors, or a diffractive optical element (DOE). In particular, we present our recent work on the development of an MMM using a spatial light modulator (SLM).

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    JUNLE QU, LIXIN LIU, YONGHONG SHAO, HANBEN NIU, BRUCE Z. GAO. RECENT PROGRESS IN MULTIFOCAL MULTIPHOTON MICROSCOPY[J]. Journal of Innovative Optical Health Sciences, 2012, 5(3): 1250018

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    Paper Information

    Received: Jun. 19, 2012

    Accepted: --

    Published Online: Jan. 10, 2019

    The Author Email: QU JUNLE (jlqu@szu.edu.cn)

    DOI:10.1142/s1793545812500186

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