A zinc ion fluorescence sensor with imaging was fabricated by using 1-(2-Pyridylazo)-2-naphthol and fluorescent reagent of rhodamine B together as the indication which was immobilized on an tip of gradient index lens by base catalyzed sol-gel method. Combined with improved metallographic microscope and laser, the measurement of zinc ion concentration and the view of sample morphology could be realized simultaneously by this system. The sensor has the linear range of zinc ion concentration from 0.1 to 1 mmol·L-1. Moreover, it has the capabilities of imaging with the whole system’s resolution of 18.73 lp·mm-1. This sensor with the function of imaging has the potential applications in biosensing.