A preparation method for diffusers with surface disordered submicron structures was proposed, which are characterized by a higher haze property and higher transmittance. By replication method, the diffusers with surface disordered submicron structures were prepared based on silicon-cone molds and anodic aluminum oxide molds for polydimethylsiloxane (PDMS) and polystyrene (PS). It demonstrates that the surface disordered submicron structures significantly enhance the haze properties of polymer films. Moreover, the morphologies and dimensions of the submicron structures have important effects on the diffusing property of the polymer films. The fabricated PDMS films with disordered submicron-holes replicated from a silicon-cone mold with a cone diameter of 650 nm has a haze of 92%, a transmittance of 90.9% and an effective diffusion coefficient of 83.7%. Accordingly, the haze, transmittance and effective diffusion coefficient of PS films obtained from the same silicon-cone mold are 97.9%, 85% and 83.2%, respectively. Both films diffuse light effectively and are used as diffusers properly. The results indicate that using silicon-cone mold based replication method to fabricate the efficient diffusers is simple, low-cost and compatible with different polymers, and is able to be employed for mass production.