As one of most important atmospheric turbulence simulator, hot air convection atmospheric turbulence simulator has been widely used in many areas. It can simulate scintillation, phase fluctuations, angle-of-arrival, beam expander and beam wander caused by atmospheric turbulence. 532, 808, 1064, 1550 nm lasers transmiting, receiving and measuring device have been used to calibrate the coherence length, refractive index structure constant, equivalent scintillation index,and the spectral characteristics and probability density distribution characteristics of the scintillation and the angle-of-arrival. Experimental results show that the range of atmospheric turbulence coherence length obtained by atmospheric turbulence simulator is 5~20 cm. The maximum C2n simulated by this simulator is 1.81×10-16 when equivalent turbulence link length is 1 km. The maximum C2n is 1.81×10-15when equivalent turbulence link length is 10 km, and the equivalent distance of scintillation index is 661.2 m. Besides, the spectral characteristics and probability density distribution characteristics of scintillation index and angle-of-arrival are similar to the real atmospheric turbulence. In summary, the atmospheric turbulence simulated by the hot air convection turbulence simulator is close to the real atmosphere.