PhotoniX, Volume. 1, Issue 1, 9(2020)

Cellular-resolution in vivo tomography in turbid tissue through digital aberration correction

En Bo1、†, Xin Ge1、†, Yuemei Luo1, Xuan Wu1, Si Chen1, Haitao Liang1, Shufen Chen1, Xiaojun Yu2, Ping Shum1, Jianhua Mo3, Nanguang Chen4, and Linbo Liu1,5、*
Author Affiliations
  • 1School of Electrical and Electronic Engineering, Nanyang Technological University, 50 Nanyang Avenue, Singapore 639798, Republic of Singapore
  • 2School of Automation, Northwestern Polytechnical University, Xi’an, 710072 Shaanxi, China
  • 3School of Electronics and Information Engineering, Soochow University, Shizi Street 1, Suzhou 215006, China
  • 4Department of Biomedical Engineering, National University of Singapore, Singapore 117583, Republic of Singapore
  • 5School of Chemical and Biomedical Engineering, Nanyang Technological University, 62 Nanyang Drive, Singapore 637459, Republic of Singapore
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    Noninvasive tomographic imaging of cellular processes in vivo may provide valuable cytological and histological information for disease diagnosis. However, such strategies are usually hampered by optical aberrations caused by the imaging system and tissue turbidity. State-of-the-art aberration correction methods require that the light signal be phase stable over the full-field data acquisition period, which is difficult to maintain during dynamic cellular processes in vivo. Here we show that any optical aberrations in the path length difference (OPD) domain can be corrected without the phase stability requirement based on maximum intensity assumption. Specifically, we demonstrate a novel optical tomographic technique, termed amplitude division aperture synthesis optical coherence tomography (ADAS-OCT), which corrects aberrations induced by turbid tissues by physical aperture synthesis and simultaneously data acquisition from sub-apertures. Even with just two sub-apertures, ADAS-OCT enabled in vivo visualization of red blood cells in human labial mucosa. We further demonstrated that adding sub-apertures could significantly scale up the aberration correction capability. This technology has the potential to impact a number of clinical areas where noninvasive examinations are preferred, such as blood count and cancers detection.

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    En Bo, Xin Ge, Yuemei Luo, Xuan Wu, Si Chen, Haitao Liang, Shufen Chen, Xiaojun Yu, Ping Shum, Jianhua Mo, Nanguang Chen, Linbo Liu. Cellular-resolution in vivo tomography in turbid tissue through digital aberration correction[J]. PhotoniX, 2020, 1(1): 9

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    Paper Information

    Category: Research Articles

    Received: Jul. 26, 2019

    Accepted: Feb. 20, 2020

    Published Online: Jul. 10, 2023

    The Author Email: Liu Linbo (liulinbo@ntu.edu.sg)

    DOI:10.1186/s43074-020-00009-7

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